Hot
- Description
Description
| Description: | LYVE-1 has been identified as a major receptor for HA (extracellular matrix glycosaminoglycan hyaluronan) on the lymph vessel wall. The deduced amino acid sequence of LYVE-1 predicts a 322-residue type I integral membrane polypeptide 41% similar to the CD44 HA receptor with a 212-residue extracellular domain containing a single Link module, the prototypic HA binding domain of the Link protein superfamily. LYVE-1 is the first lymph-specific HA receptor to be characterized. Later, LYVE-1 was reported to express in liver sinusoid blood vessels. However, in most tissues, LYVE-1 is a specific marker for lymph vessels. This antibody was raised against the extracellular domain of mouse LYVE-1 molecule in rabbit. It works well for both frozen and paraffin sections. |
|---|---|
| Host Species: | Rabbit |
| Buffer: | PBS |
| Formulation: | Protein A purified rabbit IgG |
| Stability: | The antibody is stable for at least six weeks at 2-4°C. It should be kept at –20°C for long-term storage. Avoid repeated freeze-thaw cycles. |
| Cross-Reactivity: | The antibody has not been shown to cross-react with LYVE-1 from other species. |
| Specificity: | The antibody will detect mouse LYVE-1 on the surface of lymphatic endothelial cells by immunohistochemistry (frozen and paraffin sections). The antibody detects a 70 KD LYVE-1 band in Western blotting. |
| Immunohistochemistry: | Use at 5-10 µg/ml. A boiling/microwave step is recommended when staining paraffin embedded sections. |
| Western blotting: | Use at a concentration of 1-2 µg/ml with the appropriate secondary reagents. |
| Usage: | This antibody is offered for research use only. |